The Serb faces deportation after his visa was cancelled for a second time, with the government labelling the 34-year-old a threat to public health.
His lawyers are appealing against what they called an “irrational” decision, with the hearing set for Sunday.
Djokovic is still scheduled to play the Australian Open on Monday in Melbourne.
If he were to win the tournament, he would become the most successful men’s tennis player in the history of the sport with 21 major titles.
But Sunday’s hearing, scheduled for 09:30 local time (22:30 GMT on Saturday), is crucial if Djokovic is to be able to compete just hours later.
If he loses the appeal, the world’s top-ranked men’s tennis player faces deportation and a three-year visa ban.
On Saturday, shortly after an online pre-trial hearing, Djokovic returned to the immigration detention hotel where he was held earlier this week. He will remain there until his appeal on Sunday.
Djokovic’s visa was first revoked shortly after his arrival in Melbourne on 6 January, after Australian Border Force officials said he had “failed to provide appropriate evidence” to receive a vaccine exemption.
The tennis star, who has caught Covid-19 twice with the latest positive test on 16 December, was detained for days at the immigration hotel.
His visa was then reinstated by a judge, who ordered his release, ruling that border officials had ignored correct procedure when he arrived.
But on Friday evening, Immigration Minister Alex Hawke once again cancelled Djokovic’s visa under separate powers in Australia’s Migration Act.
The act allows him to deport anyone he deems a potential risk to “the health, safety or good order of the Australian community”.
Prime Minister Scott Morrison said the decision had followed “careful consideration”.
Alluding to the heavy criticism his government has faced for allowing the unvaccinated player into Australia, Mr Morrison said: “Australians have made many sacrifices during this pandemic, and they rightly expect the result of those sacrifices to be protected.”
Minutes after we started reporting the news of Djokovic’s second visa cancellation, motorists leaned from car windows, honking their horns and shouting their approval.
Most people I’ve spoken to are pretty outraged that an unvaccinated player was ever allowed here in the first place.
Others have a more nuanced view. Sure, this is a shambles, they say, but the government overturning the ruling of an independent judge is pretty questionable too.
It’s clear, then, that this is not just about sport. It’s headline news and a major topic of conversation.
And the background is important, too, as Australia struggles with Covid-19.
Many people are getting jabbed after months of living under strict restrictions. Intensive care wards are filling up, daily deaths have hit record levels, and some might say you’ve got less chance of finding a Covid test kit in a pharmacy than Djokovic has of playing on Monday.
Given the seriousness of the Omicron wave, there’s another sentiment that you hear often: this drawn-out saga has become a distraction from far more important issues.
Court documents released on Saturday show Mr Hawke chose to cancel Djokovic’s visa because – in his view – the unvaccinated player’s presence could fuel opposition to Covid-19 vaccination.
“[I] consider that his presence may be a risk to the health of the Australian community,” he wrote in a letter to Djokovic and his lawyers, adding that he believed it could also provoke “civil unrest” because he was “a person of influence and status”.
Djokovic’s legal team say their grounds for appeal will centre on the “invalid and illogical” rationale of Mr Hawke’s decision, which lawyer Nick Wood said was based on the threat of “exciting anti-vax sentiment”.
Mr Wood said he believed deporting the player would potentially do the same thing.
Serbian President AleksandarVucic condemned the Australian minister’s decision, telling Djokovic in an Instagram message: “Novak, we stand by you.”
“If you wanted to ban Novak Djokovic from winning the 10th trophy in Melbourne why didn’t you return him immediately, why didn’t you tell him ‘it is impossible to obtain a visa’?” MrVucic added.
Spanish tennis star Rafael Nadal, one of Djokovic’s biggest rivals, said on Saturday: “[The] Australian Open is much more important than any player. If he’s playing finally, okay. If he’s not playing, the Australian Open will be great… with or without him.”
Japanese player Naomi Osaka described the controversy surrounding Djokovic as “an unfortunate situation”.
“He’s such a great player and it’s kind of sad that some people might remember [him] in this way. But I also think it’s… up to the government how Australia is deciding to handle it,” she said.